Cancer Research Malaysia offer the analysis of non-volatile small molecules (mass ranging from 100 to 1500 dalton) using a hyphenated technique Liquid Chromatography Mass Spectrometry (LCMS) on the AcquityTM Waters Ultra Performance Liquid Chromatography and Synapt Q-TOF Mass Spectrometer.  This service is open for any researcher with research focuses on natural products and small molecules molecular weight analysis.

  • There are 2 main types of sample primary analysis, where researcher needs to identify either a nominal or an accurate mass analysis is needed. The prices are varied according to the type of samples on the selected primary analysis, as shown in the table below. If further mass fragmentation analysis (MS/MS) or HPLC method development is needed, an additional add-on fee is required.

    • lcmsprice
    • What system does Cancer Research Malaysia use?
    1. Instrument details: AcquityTM Waters Ultra Performance Liquid Chromatography (UPLC) (Waters Corporation, USA)
    2. Software used: Waters MassLynx 4.1 Column specification: ACQUITY UPLC BEH C18 1.7µm, 2.1 x 50mm Column
    3. Detector: ACQUITY PDA Detector (ACQ-PDA) Version 1.40.1932 Mass
    4. Spectrometer: Synapt High Definition Mass Spectrometer quadrupole-orthogonal acceleration, time-of-flight detector (Waters Corporation, USA) Electrospray ionization (ESI) Source (same for +ve and –ve mode) (different supplier named this differently):
    1. a) Capillary voltage: 2.7kV
    2. b) Sampling cone: 40
    3. c) Extraction cone: 4.0
    4. d) Source temperature: 100 °C
    5. e) Desolvation temperature: 350°C
    6. f) Cone gas flow: 30 L/h
    7. g) Desolvation gas flow: 700 L/h Water used: MilliQ water (Millipore Bedford, MA, USA) Solvent used are LCMS Optima grade.
    • What is the difference between Nominal and Accurate run?
      During accurate run, a concurrent lock-mass analysis is conducted for mass correction. The generated mass value (in four decimal places) is available for molecular formula analysis. A nominal run is conducted without the concurrent run of lock-mass analysis.
    • Which mode of ionization should I choose?
      It depends on the nature of the compounds in your sample. Please refer related journal for guidance.
    • Could your analysis be run at several wavelengths?
      Yes, we use Photo Diode Array (PDA) as detector which covers 190 to 800 nm.
    • What is the range of molecular weight for the sample analysis?
      100 to 1500 dalton.
    • Does Cancer Research Malaysia provide access to a compound library?
      No.
    • Which one is my parent peak? If a peak has more than one mass, does it mean it’s a mixture of compounds? And which mass should I choose to further fragment (MS/MS)?
      In most cases, the highest m/z is likely to be the parent peak, with the exception of the presence of adduct. The other masses may either be fragment ions or another parent peak, which could be confirmed by further MS/MS fragmentation analysis on both masses. It is recommended that you choose masses that are not too low that it could be mistaken as noise.
    • Do you do quantification analysis?
      No.
    • How long do I have to wait to get my LCMS report?
      Please allow minimum 30 working days for sample analysis. Shorter time analysis is subjected to machine availability and sample queue.