Analysis type |
Sample type |
Cost per service |
Primary analysis |
Nominal mass (Positive / negative ionisaation) |
Pure |
RM 325 |
Crude |
RM 480 |
Accurate mass (Positive / negative ionisation) |
Pure |
RM 380 |
Crude |
RM 590 |
Add-on analysis (if required) |
MS/MS for every 5 major peaks |
Pure / Crude |
RM 275 |
HPLC method development |
RM 190 |
Analysis type |
Sample type |
Cost per service |
Nominal mass (Positive / negative ionisation) |
Pure |
RM 325 |
Crude |
RM 480 |
Accurate mass (Positive / negative ionisation) |
Pure |
RM 380 |
Crude |
RM 590 |
MS/MS for every 5 major peaks |
Pure / Crude |
RM 275 |
HPLC method development |
RM 190 |
Faq:
What’s the difference between the nominal and accurate run?
During an accurate run, a concurrent lock-mass analysis is conducted for mass correction. The generated mass value (in 4 decimal places) will then be available for molecular formula analysis. A nominal run is conducted without the lock-mass analysis.
Which mode of ionisation should I use?
It depends on the nature of the compounds in your sample. Please refer to the related journals for guidance.
Can the analysis be run at several wavelengths?
Yes, we use the Photo Diode Array (PDA) as a detector, which covers 190nm – 800 nm.
Which is my parent peak? If a peak has >1 mass, does it mean it’s a mixture of compounds? Which mass should I choose to further fragment (MS/MS)?
The highest m/z is likely to be the parent peak, with the exception of the presence of adduct. The other masses may either be fragment ions or another parent peak, which can be confirmed by further MS/MS fragmentation analysis on both masses. It’s recommended that you choose masses that aren’t too low to not mistake them as noise.
When will my LCMS report be ready?
Please allow a minimum of 30 working days for sample analysis, subjected to machine availability and sample queue.
Does CRM conduct quantification analysis or provide access to a compound library?
No, we do not.
Technical specifications:
|
Specifications |
Instrument details |
Acquity Waters Ultra Performance Liquid Chromatography (UPLC) (Waters Corporation, USA) |
Software used |
Waters MassLynx 4.1 Column specification |
Detector |
ACQUITY PDA Detector (ACQ-PDA) Version 1.40.1931 Mass ACQUITY UPLC BEH C18 1.7µm, 2.1 x 50mm Column |
Spectrometer |
Synapt High Definition Mass Spectrometer quadrupole-orthogonal acceleration, time-of-flight detector (Waters Corporation, USA) Electrospray ionization (ESI) Source (same for +ve and –ve mode) (name varies by supplier) |
Capillary voltage: 2.7 kV
Sampling cone: 40
Extraction cone: 4.0
Source temperature: 100°C
Desolvation temperature: 350°C
Cone gas flow: 30 L/h
Desolvation gas flow: 700 L/h
|
Water used |
MilliQ water (Millipore Bedford, MA, USA) Solvent used are LCMS Optima grade |
For more information, please contact Mdm Norazwana Samat at
services@cancerresearch.my or +603-2712 3224