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Services

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Lcms

We offer analysis of non-volatile small molecules (mass ranging from 100-1500 dalton) using a hyphenated technique called Liquid Chromatography Mass Spectrometry (LCMS) on the Acquity^TM Water Ultra Performance Liquid Chromatography and Synapt Q-TOF Mass Spectrometer.

This service is available to any researcher whose focus in on natural products and small molecules’ molecular weight analysis.

There are 2 main types of sample primary analysis, where researchers need to identify whether a nominal or accurate mass analysis is required. The prices vary according to the type of samples with the selected primary analysis. If further mass fragmentation analysis (MS/MS) or the HPLC method development is needed, an additional fee will be charged.

Analysis type		Sample type	Cost per service
Primary analysis	Nominal mass (Positive / negative ionisaation)	Pure	RM 325
	Nominal mass (Positive / negative ionisaation)	Crude	RM 480
	Accurate mass (Positive / negative ionisation)	Pure	RM 380
	Accurate mass (Positive / negative ionisation)	Crude	RM 590
Add-on analysis (if required)	MS/MS for every 5 major peaks	Pure / Crude	RM 275
Add-on analysis (if required)	HPLC method development	Pure / Crude	RM 190

Analysis type	Sample type	Cost per service
Primary analysis
Nominal mass (Positive / negative ionisation)	Pure	RM 325
Nominal mass (Positive / negative ionisation)	Crude	RM 480
Accurate mass (Positive / negative ionisation)	Pure	RM 380
Accurate mass (Positive / negative ionisation)	Crude	RM 590
Add-on analysis (if required)
MS/MS for every 5 major peaks	Pure / Crude	RM 275
HPLC method development	Pure / Crude	RM 190

Faq:

What’s the difference between the nominal and accurate run?

During an accurate run, a concurrent lock-mass analysis is conducted for mass correction. The generated mass value (in 4 decimal places) will then be available for molecular formula analysis. A nominal run is conducted without the lock-mass analysis.

Which mode of ionisation should I use?

It depends on the nature of the compounds in your sample. Please refer to the related journals for guidance.

Can the analysis be run at several wavelengths?

Yes, we use the Photo Diode Array (PDA) as a detector, which covers 190nm – 800 nm.

Which is my parent peak? If a peak has >1 mass, does it mean it’s a mixture of compounds? Which mass should I choose to further fragment (MS/MS)?

The highest m/z is likely to be the parent peak, with the exception of the presence of adduct. The other masses may either be fragment ions or another parent peak, which can be confirmed by further MS/MS fragmentation analysis on both masses. It’s recommended that you choose masses that aren’t too low to not mistake them as noise.

When will my LCMS report be ready?

Please allow a minimum of 30 working days for sample analysis, subjected to machine availability and sample queue.

Does CRM conduct quantification analysis or provide access to a compound library?

No, we do not.

Technical specifications:

	Specifications
Instrument details	Acquity Waters Ultra Performance Liquid Chromatography (UPLC) (Waters Corporation, USA)
Software used	Waters MassLynx 4.1 Column specification
Detector	ACQUITY PDA Detector (ACQ-PDA) Version 1.40.1931 Mass ACQUITY UPLC BEH C18 1.7µm, 2.1 x 50mm Column
Spectrometer	Synapt High Definition Mass Spectrometer quadrupole-orthogonal acceleration, time-of-flight detector (Waters Corporation, USA) Electrospray ionization (ESI) Source (same for +ve and –ve mode) (name varies by supplier)
Spectrometer	Capillary voltage: 2.7 kV Sampling cone: 40 Extraction cone: 4.0 Source temperature: 100°C Desolvation temperature: 350°C Cone gas flow: 30 L/h Desolvation gas flow: 700 L/h
Water used	MilliQ water (Millipore Bedford, MA, USA) Solvent used are LCMS Optima grade

For more information, please contact Mdm Norazwana Samat at services@cancerresearch.my or +603-2712 3224